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A sequence of 11 consecutive arginine residues (11R) is one of the best protein transduction domains for introducing proteins into cell membranes. Heme-oxygenase-1 (HO-1) is involved in heme catabo...

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Sep 01, 2010·Thin-layer chromatography (TLC) plates made of polyethylene-imine (PEI; thickness, 0.1 mm) cellulose were from Macherey-Nagel (Düren, Germany). T4 polynucleotide kinase was from USB (Cleveland, OH, USA), γ-32 P-ATP (3000 Ci/mmol, 10 μCi/μL) was from GE Healthcare (Little Chalfont, UK). Propidium iodide was from AppliChem GmbH (Darmstadt ...

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The synthesis route of 8BSH-11R is shown in Fig. 2. The structure contains multiple boron clusters and one CPP, and such a dendritic structure containing one CPP has seldom been reported [27] . ...

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(PDF) Protein therapy using heme-oxygenase-1 fused to a ...

Jun 08, 2011·muscle cells (C) were incubated with 11R, 11R-EGFP, 11R-HO-1, or 1% Triton X. Graph shows that 11R, 11R-EGFP, and 11R-HO- 1 did not affect cell growth and have little toxicity in vitro .

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(PDF) Protein therapy using heme-oxygenase-1 fused to a ...

Jun 08, 2011·muscle cells (C) were incubated with 11R, 11R-EGFP, 11R-HO-1, or 1% Triton X. Graph shows that 11R, 11R-EGFP, and 11R-HO- 1 did not affect cell growth and have little toxicity in vitro .

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Protein therapy using heme-oxygenase-1 fused to a ...

Jun 08, 2011·Previously, we constructed a pET-21a(+)-p53-11R plasmid from pET-21a(+)-11R. This pET-21a(+)-11R was produced by modification of a pET-21a(+) vector (Novagen, Madison, WI, USA) (Takenobu et al, 2002). In brief, the vector was tagged with a 6-histidine leader, followed by an 11-arginine flanked by glycine and glutamic acid residues (for free ...

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Protein therapy using heme-oxygenase-1 fused to a ...

Jun 08, 2011·Previously, we constructed a pET-21a(+)-p53-11R plasmid from pET-21a(+)-11R. This pET-21a(+)-11R was produced by modification of a pET-21a(+) vector (Novagen, Madison, WI, USA) (Takenobu et al, 2002). In brief, the vector was tagged with a 6-histidine leader, followed by an 11-arginine flanked by glycine and glutamic acid residues (for free ...

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May 01, 2006·Poly-Arg peptide (11R) has also been reported to exhibit enhanced protein transduction abilities , , . However, as is shown in Fig. 1C, CTP512 proved much more potent than poly-Arginine (CTP514) with regard to protein transduction capacity. Download : Download full-size image; Fig. 1. Analysis of CTP candidates with regard to their transduction ...

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(PDF) Alexander von Humboldt, die Sklavereien in den ...

This is the introduction to Alexander von Humboldts "lost diary" (Havana 1804), in German. This diary has until today never been published/ Esto es la introducción al "diario perdido" de ...

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Academia.edu is a platform for academics to share research papers.

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